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Animal Cell Biotechnology
Methods and Protocols
Taschenbuch von Ralf Pörtner
Sprache: Englisch

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Beschreibung
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis
Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation.- Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays.- Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment.- ambr® 15 Microbioreactors for CHO Cell Cultivation.- Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications.- HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR).- Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode.- Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors.- Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production.- How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.- Generic Workflow for the Setup of Mechanistic Process Models.- Estimation of Process Model Parameters.- Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments.- Design, Optimization, and Adaptive Control of Cell Culture Seed Trains.- High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays.- Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture.- Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells.- Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples.- An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures.- Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures.- Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method.- Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System.
Details
Erscheinungsjahr: 2021
Fachbereich: Gentechnologie
Genre: Biologie
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
Reihe: Methods in Molecular Biology
Inhalt: xiv
399 S.
47 s/w Illustr.
80 farbige Illustr.
399 p. 127 illus.
80 illus. in color.
ISBN-13: 9781071601938
ISBN-10: 1071601938
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Redaktion: Pörtner, Ralf
Herausgeber: Ralf Pörtner
Auflage: 4th ed. 2020
Hersteller: Springer US
Springer New York
Springer US, New York, N.Y.
Methods in Molecular Biology
Maße: 254 x 178 x 23 mm
Von/Mit: Ralf Pörtner
Erscheinungsdatum: 17.01.2021
Gewicht: 0,779 kg
Artikel-ID: 119393699
Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis
Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation.- Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays.- Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment.- ambr® 15 Microbioreactors for CHO Cell Cultivation.- Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications.- HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR).- Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode.- Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors.- Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production.- How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale.- Generic Workflow for the Setup of Mechanistic Process Models.- Estimation of Process Model Parameters.- Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments.- Design, Optimization, and Adaptive Control of Cell Culture Seed Trains.- High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays.- Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture.- Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells.- Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples.- An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures.- Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures.- Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method.- Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System.
Details
Erscheinungsjahr: 2021
Fachbereich: Gentechnologie
Genre: Biologie
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
Reihe: Methods in Molecular Biology
Inhalt: xiv
399 S.
47 s/w Illustr.
80 farbige Illustr.
399 p. 127 illus.
80 illus. in color.
ISBN-13: 9781071601938
ISBN-10: 1071601938
Sprache: Englisch
Ausstattung / Beilage: Paperback
Einband: Kartoniert / Broschiert
Redaktion: Pörtner, Ralf
Herausgeber: Ralf Pörtner
Auflage: 4th ed. 2020
Hersteller: Springer US
Springer New York
Springer US, New York, N.Y.
Methods in Molecular Biology
Maße: 254 x 178 x 23 mm
Von/Mit: Ralf Pörtner
Erscheinungsdatum: 17.01.2021
Gewicht: 0,779 kg
Artikel-ID: 119393699
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