Dr. Shalini Mani is Associate Professor in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Manisha Singh is Assistant Professor and Lecturer in the Department of Biotechnology at Jaypee Institute of Information Technology, Noida, India. Dr. Anil Kumar is Director of Education and Professor at RLB Central Agricultural University, Jhansi, India.

Includes best practices and lab safety, ethical considerations, and troubleshooting for common issues

Perfect for junior researchers, research students, and established scientists seeking a go-to quick guide

One-stop reference or standard animal cell culture techniques

1. Overview to animal cell culture.- 2. Cell culture laboratory.- 3. Good lab practices and bio-safety measures.- 4. Managing sterility in the lab.- 5. Media and buffer preparation for cell culture.- 6. Properties of cultured cells and selection of culture media.- 7. Selection and maintenance of cultured cells.- 8. Inoculation and passaging of adherent cells.- 9. Counting of cells.- 10. Cryopreservation of cell lines.- 11. Resuscitation of frozen cell line.- 12. Isolation and culturing of cells from different tissues.- 13. Differentiation, transformation and immortalization of cells.- 14. Co-culture techniques.- 15. 3d cell culture techniques.- 16. Stem cell culture techniques.- 17. Identification and removal of biological contamination in the media and cell suspensions.- 18. Analysis of cell growth kinetics in suspension and adherent types cell line.- 19. Applications of animal cell culture based assays.- 20. Ethical issues in animal cell culture.- 21. Common troubleshooting methods in cell culture techniques.