Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Basic Phage Mathematics.- Analysis of Host-Takeover during SPOi Infection of
Bacillus subtilis.- P
ractical Advice on the One-Step Growth Curve.- Iron Chloride Flocculation of Bacteriophages from Seawater.- Purification of Bacteriophages using Anion-Exchange Chromatography.- Encapsulation Strategies of Bacteriophages (Felix O1) for Oral Therapeutic Application.- Encapsulation of
Listeria
Phage A511 by Alginate to Improve its Thermal Stability.- Application of a Virucidal Agent to Avoid Over-Estimation of Phage Kill during Phage Decontamination Assays on Ready-to-Eat Meats.- Sequencing, Assembling, and Finishing Complete Bacteriophage Genomes.- Identification of DNA Base Modifications by Means of Pacific Biosciences RS Sequencing Technology.- Analyzing Genome Termini of Bacteriophage Through High-Throughput Sequencing.- Amplification for Whole Genome Sequencing of Bacteriophages from Single Isolated Plaques using SISPA.- Genome Sequencing of dsDNA-Containing Bacteriophages Directly from a Single Plaque.- Preparing cDNA Libraries from Lytic Phage Infected Cells for Whole Transcriptome Analysis by RNA-Seq.- Essential Steps in Characterizing Bacteriophages: Biology, Taxonomy, and Genome Analysis.- Annotation of Bacteriophage Genome Sequences using DNA Master: An Overview.- Phage Genome Annotation using the RAST Pipeline.- Visualization of Phage Genomic Data: Comparative Genomics and Publication-Quality Diagrams.- Transposable Bacteriophages as Genetic Tools.- Applications of the Bacteriophage Mu In Vitro Transposition Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes.- Use of RP4::mini-Mu for Gene Transfer.- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu.