Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-Borne Pathogens in 48 or 96 Samples.- Direct Detection of Borrelia Species in Tissues.- Purification of Borrelia burgdorferi Outer Membrane Vesicles.- Constructing an ELISA for Detection of Anti-Borrelia in Wildlife and Agricultural Animals.- A Step-by-Step Guide to Selecting an Optimal Cut-Off Value Based on the Receiver Operating Characteristic and Youden Index in Methods Designed to Diagnose Lyme Disease.- Adaptive Immune Response Investigation in Lyme borreliosis.- CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, An Alternative to LYMESPOT.- Use of Specific Borrelia Phages as a New Strategy for Improved Diagnostic Tests.- Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass Spectrometry.- Preparation of Borrelia Infected Mammalian Cells for Helium Ion Microscopy.- Establishing a Zebrafish Model for Borrelia Burgdorferi Infection Using Immersion and Microinjection Methods.- Development of Organoids to Study Infectious Host Interactions.- Content Analysis: An Approach to Exploring the Depiction of Tick-Borne Diseases.- Applying BERT and ChatGPT for Sentiment Analysis of Lyme Disease in Scientific Literature.- Building a Binary Classification Machine Learning Model: A Guide to Predicting Participation in a Lyme Disease Program at a Medical Institute.- Clinical Algorithm and Diagnostic Tools for Lyme Disease.- Establishing a Pregnancy Lyme Disease Biobank.- Developing a Prospective Gestational Lyme Disease Study.