Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Investigating CRISPR RNA Biogenesis and Function using RNA-seq.- In vitro Co-reconstitution of Cas Protein Complexes.- Analysis of CRISPR pre-crRNA Cleavage.- Annotation and Classification of CRISPR-Cas Systems.- Computational Detection of CRISPR/crRNA Targets.- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium.- Spacer-based Macroarrays for CRISPR Genotyping.- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS).- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR.- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli.- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes.- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids .- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition.- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl.- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates.- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems.- Cas3 Nuclease-Helicase Activity Assays.- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex.- Creation andAnalysis of a Virome: Using CRISPR Spacers.- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases.- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9.- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).