Foreword Alan Trounson; Part I. Culture Media and Solutions: 1. Overview Patrick Quinn; 2. Media and embryo interactions Patrick Quinn; 3. Female tract environment and its relationship to ART media composition Henry J. Leese and Sarah Louise Whitear; 4. Buffering systems in IVF Natalie A. Clark and Jason E. Swain; 5. Essential features in media development for spermatozoa, oocytes and embryos David Mortimer; 6. Macromolecular supplementation of embryo culture media Thomas B. Pool; 7. Monozygotic twinning and assisted reproduction. Laboratory or clinical? Marius Meintjes; 8. Amino acids and ammonium Deirdre Zander-Fox and Michelle Lane; 9. Growth factors and cytokines in embryo development Sarah A. Robertson and Jeremy G. Thompson; 10. Osmolality Jay M. Baltz; 11. pH control in the embryo culture environment Joe Conaghan; 12. In vitro maturation of immature human oocytes Ri-Cheng Chian, Shan-Jun Dai and Yao Wang; 13. Oocyte and embryo cryopreservation media Diana Patricia Bernal, Ching-Chien Chang and Zsolt Peter Nagy; 14. International regulation of ART media Theresa Jeary; Part II. Culture Systems: 15. Culture of embryos in dynamic fluid environments André Monteiro da Rocha and Gary Daniel Smith; 16. Time-lapse imaging of embryo development: using morphokinetic analysis to select viable embryos Markus H. M. Montag, Kamilla S. Pedersen and Niels B. Ramsing; 17. Optimizing culture conditions Kathleen A. Miller; 18. Low cost IVF Luca Gianaroli, M. Cristina Magli and Anna P. Ferraretti; 19. Incubators old and new Eduardo Kelly and Takeo Cho; 20. Incubator management William R. Boone and H. Lee Higdon, III; 21. Summary and conclusions Patrick Quinn; Index.