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Digital PCR
Methods and Protocols
Buch von Francisco Bizouarn (u. a.)
Sprache: Englisch

258,95 €*

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Beschreibung
This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.¿
This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.¿
Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis
Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR.- Basic Concepts and Validation of Digital PCR Measurements.- Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean?.- Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from Plasma.- Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events.- Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples.- Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR.- Testing of General and Human-Associated Fecal Contamination in Waters.- Analyzing Copy Number Variation with Droplet Digital PCR.- Assessing HER2 Amplification in Plasma cfDNA.- Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues using ddPCR: analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants.- Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer using Droplet Digital PCR.- Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients.- Droplet Digitial PCR for Miniaml Residual Disease Detection in Mature Lymphoproliferative Disorders.- Quantitation of JAK2 V617F Allele Burden by using the QuantStudio(TM) 3D Digitial PCR System.- Novel Multiplexing Strategies for Quantification of Rare Alleles using ddPCR.- Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA.- Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.- A Universal Droplet Digital PCR Approach for Monitoring of Graft Health after Transplantation using a Pre-Selected SNP Set.- Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci using Droplet Digital PCR.- DNA Methylation Analysis Using Droplet Digital PCR.- Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts using Droplet Digital PCR.- Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression.- Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCR(TM) Assays.- Quantification of Circulating MicroRNAs by Droplet Digital PCR using EvaGreen Probeless Chemistry.- Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker has-miR-141.- Droplet Digital(TM) PCR Next-Generation Sequencing Library QC Assay.- Phasing DNA Markers using Digital PCR.- ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity.- Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method.
Details
Erscheinungsjahr: 2018
Fachbereich: Andere Fachgebiete
Genre: Importe, Medizin
Rubrik: Wissenschaften
Medium: Buch
Reihe: Methods in Molecular Biology
Inhalt: xv
557 S.
13 s/w Illustr.
128 farbige Illustr.
557 p. 141 illus.
128 illus. in color.
ISBN-13: 9781493977765
ISBN-10: 1493977768
Sprache: Englisch
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Redaktion: Bizouarn, Francisco
Karlin-Neumann, George
Herausgeber: George Karlin-Neumann/Francisco Bizouarn
Auflage: 1st ed. 2018
Hersteller: Springer New York
Springer US, New York, N.Y.
Methods in Molecular Biology
Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße: 260 x 183 x 37 mm
Von/Mit: Francisco Bizouarn (u. a.)
Erscheinungsdatum: 01.05.2018
Gewicht: 1,266 kg
Artikel-ID: 110859608
Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis
Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR.- Basic Concepts and Validation of Digital PCR Measurements.- Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean?.- Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from Plasma.- Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events.- Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples.- Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR.- Testing of General and Human-Associated Fecal Contamination in Waters.- Analyzing Copy Number Variation with Droplet Digital PCR.- Assessing HER2 Amplification in Plasma cfDNA.- Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues using ddPCR: analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants.- Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer using Droplet Digital PCR.- Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients.- Droplet Digitial PCR for Miniaml Residual Disease Detection in Mature Lymphoproliferative Disorders.- Quantitation of JAK2 V617F Allele Burden by using the QuantStudio(TM) 3D Digitial PCR System.- Novel Multiplexing Strategies for Quantification of Rare Alleles using ddPCR.- Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA.- Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.- A Universal Droplet Digital PCR Approach for Monitoring of Graft Health after Transplantation using a Pre-Selected SNP Set.- Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci using Droplet Digital PCR.- DNA Methylation Analysis Using Droplet Digital PCR.- Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts using Droplet Digital PCR.- Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression.- Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCR(TM) Assays.- Quantification of Circulating MicroRNAs by Droplet Digital PCR using EvaGreen Probeless Chemistry.- Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker has-miR-141.- Droplet Digital(TM) PCR Next-Generation Sequencing Library QC Assay.- Phasing DNA Markers using Digital PCR.- ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity.- Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method.
Details
Erscheinungsjahr: 2018
Fachbereich: Andere Fachgebiete
Genre: Importe, Medizin
Rubrik: Wissenschaften
Medium: Buch
Reihe: Methods in Molecular Biology
Inhalt: xv
557 S.
13 s/w Illustr.
128 farbige Illustr.
557 p. 141 illus.
128 illus. in color.
ISBN-13: 9781493977765
ISBN-10: 1493977768
Sprache: Englisch
Ausstattung / Beilage: HC runder Rücken kaschiert
Einband: Gebunden
Redaktion: Bizouarn, Francisco
Karlin-Neumann, George
Herausgeber: George Karlin-Neumann/Francisco Bizouarn
Auflage: 1st ed. 2018
Hersteller: Springer New York
Springer US, New York, N.Y.
Methods in Molecular Biology
Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße: 260 x 183 x 37 mm
Von/Mit: Francisco Bizouarn (u. a.)
Erscheinungsdatum: 01.05.2018
Gewicht: 1,266 kg
Artikel-ID: 110859608
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