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The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, [...] Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at [...] Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013).
> 250, H=30, cit > 4000 (Google Scholar). AD isinvolved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects.
Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to 'Microscopy, Research and Technique (Wiley).
A comprehensive text that starts from the fundamental optical imaging concepts
Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques
An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology
A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics
Contains three useful Appendices
Lists of recommended books at the end of each chapter, to expand on various specialized topics
Written by two acknowledged experts in this rapidly evolving, interdisciplinary field
Includes supplementary material: [...]
| Erscheinungsjahr: | 2014 |
|---|---|
| Fachbereich: | Mechanik & Akustik |
| Genre: | Physik |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Buch |
| Inhalt: |
xv
218 S. 85 s/w Illustr. 55 farbige Illustr. 218 p. 140 illus. 55 illus. in color. |
| ISBN-13: | 9789400775442 |
| ISBN-10: | 940077544X |
| Sprache: | Englisch |
| Herstellernummer: | 86061629 |
| Ausstattung / Beilage: | HC runder Rücken kaschiert |
| Einband: | Gebunden |
| Autor: |
Diaspro, Alberto
Mondal, Partha Pratim |
| Hersteller: |
Springer Netherland
Springer Netherlands |
| Maße: | 285 x 215 x 19 mm |
| Von/Mit: | Alberto Diaspro (u. a.) |
| Erscheinungsdatum: | 08.01.2014 |
| Gewicht: | 0,84 kg |
Alberto Diaspro, born in Genoa, Italy, on April 7, 1959, received his Laurea in Electronic Engineering in 1983, [...] Genoa. Dir. Nanophys at Italian Institute of Technology (IIT), Deputy Director of IIT, Prof. Appl. Phys. at [...] Genoa. President of Optics Within Life Sciences, IEEE Senior member and Editor in chief of Microscopy Research and Technique (7/2013).
> 250, H=30, cit > 4000 (Google Scholar). AD isinvolved in research development of instrumentation for applications in biophysics and biomedical engineering and in nanobiotechnological projects.
Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to 'Microscopy, Research and Technique (Wiley).
A comprehensive text that starts from the fundamental optical imaging concepts
Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques
An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology
A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics
Contains three useful Appendices
Lists of recommended books at the end of each chapter, to expand on various specialized topics
Written by two acknowledged experts in this rapidly evolving, interdisciplinary field
Includes supplementary material: [...]
| Erscheinungsjahr: | 2014 |
|---|---|
| Fachbereich: | Mechanik & Akustik |
| Genre: | Physik |
| Rubrik: | Naturwissenschaften & Technik |
| Medium: | Buch |
| Inhalt: |
xv
218 S. 85 s/w Illustr. 55 farbige Illustr. 218 p. 140 illus. 55 illus. in color. |
| ISBN-13: | 9789400775442 |
| ISBN-10: | 940077544X |
| Sprache: | Englisch |
| Herstellernummer: | 86061629 |
| Ausstattung / Beilage: | HC runder Rücken kaschiert |
| Einband: | Gebunden |
| Autor: |
Diaspro, Alberto
Mondal, Partha Pratim |
| Hersteller: |
Springer Netherland
Springer Netherlands |
| Maße: | 285 x 215 x 19 mm |
| Von/Mit: | Alberto Diaspro (u. a.) |
| Erscheinungsdatum: | 08.01.2014 |
| Gewicht: | 0,84 kg |
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