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Gangliosides
Methods and Protocols
Buch von Alessandro Prinetti (u. a.)
Sprache: Englisch

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Beschreibung
This detailed volume explores methods of examining gangliosides, membrane amphiphilic lipids that are deeply involved in the modulation of the overall properties of cellular membranes, as well as of the functions of membrane-associated proteins. The contents take into account the vital availability of new enzymatic approaches, the knowledge of an incredibly extensive number of glyco-enzymes, and the improvement of multi-genetic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Gangliosides: Methods and Protocols serves as a valuable guidebook for entering in the vast research world of gangliosides.
This detailed volume explores methods of examining gangliosides, membrane amphiphilic lipids that are deeply involved in the modulation of the overall properties of cellular membranes, as well as of the functions of membrane-associated proteins. The contents take into account the vital availability of new enzymatic approaches, the knowledge of an incredibly extensive number of glyco-enzymes, and the improvement of multi-genetic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Gangliosides: Methods and Protocols serves as a valuable guidebook for entering in the vast research world of gangliosides.
Zusammenfassung

Includes cutting-edge techniques for the study of gangliosides

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Inhaltsverzeichnis
Chemical and Physicochemical Properties of Gangliosides.- Gangliosides of the Nervous System.- Scattering Techniques and Ganglioside Aggregates: Laser Light, Neutron and X-Ray Scattering.- Gangliosides in the Immune System: Role of Glycosphingolipids and Glycosphingolipid-Enriched Lipid Rafts in Immunological Functions.- Ganglioside Metabolism and Its Inherited Diseases.- Gangliosides and Tumors.- Anti-Ganglioside Antibodies in Peripheral Nerve Pathology.- Protocols of Glycosyltransferase Assays: Ganglioside Globoside and Lewis-X Intermediate-Lactosylceramide Biosyntheses in Eukeryotic Systems.- Mass Spectrometry of Gangliosides.- Visualization of Brain Gangliosides Using MALDI Imaging Mass Spectrometry.- Immunoelectron Microscopy of Gangliosides.- Nuclear Magnetic Resonance of Gangliosides.- Methods for the Preparation of Anti-Ganglioside Monoclonal Antibodies.- Chemical Synthesis of Gangliosides.- Radioactive Gangliosides for Biological Studies.- Synthesis of Fluorescent Gangliosides.- Synthesis of Photoactivatable and Paramagnetic Gangliosides.- Methods for Assay of Ganglioside Catabolic Enzymes.- Pharmacological Modulation of Glycosphingolipid Metabolism.- Molecular Dynamics of Gangliosides.- Determination of Glycolipid Ligands of Carbohydrate-Binding Proteins Directly on Thin Layer Chromatograms.- Identification of KDN-Gangliosides.- Glycoreplica Peptides.
Details
Erscheinungsjahr: 2018
Fachbereich: Biophysik
Genre: Biologie, Importe
Rubrik: Naturwissenschaften & Technik
Medium: Buch
Inhalt: xv
456 S.
45 s/w Illustr.
56 farbige Illustr.
456 p. 101 illus.
56 illus. in color.
ISBN-13: 9781493985517
ISBN-10: 1493985515
Sprache: Englisch
Einband: Gebunden
Autor: Sonnino, Sandro
Prinetti, Alessandro
Redaktion: Prinetti, Alessandro
Sonnino, Sandro
Herausgeber: Sandro Sonnino/Alessandro Prinetti
Auflage: 1st edition 2018
Hersteller: Springer US
Springer New York
Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße: 260 x 183 x 31 mm
Von/Mit: Alessandro Prinetti (u. a.)
Erscheinungsdatum: 21.06.2018
Gewicht: 1,078 kg
Artikel-ID: 111799935
Zusammenfassung

Includes cutting-edge techniques for the study of gangliosides

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Inhaltsverzeichnis
Chemical and Physicochemical Properties of Gangliosides.- Gangliosides of the Nervous System.- Scattering Techniques and Ganglioside Aggregates: Laser Light, Neutron and X-Ray Scattering.- Gangliosides in the Immune System: Role of Glycosphingolipids and Glycosphingolipid-Enriched Lipid Rafts in Immunological Functions.- Ganglioside Metabolism and Its Inherited Diseases.- Gangliosides and Tumors.- Anti-Ganglioside Antibodies in Peripheral Nerve Pathology.- Protocols of Glycosyltransferase Assays: Ganglioside Globoside and Lewis-X Intermediate-Lactosylceramide Biosyntheses in Eukeryotic Systems.- Mass Spectrometry of Gangliosides.- Visualization of Brain Gangliosides Using MALDI Imaging Mass Spectrometry.- Immunoelectron Microscopy of Gangliosides.- Nuclear Magnetic Resonance of Gangliosides.- Methods for the Preparation of Anti-Ganglioside Monoclonal Antibodies.- Chemical Synthesis of Gangliosides.- Radioactive Gangliosides for Biological Studies.- Synthesis of Fluorescent Gangliosides.- Synthesis of Photoactivatable and Paramagnetic Gangliosides.- Methods for Assay of Ganglioside Catabolic Enzymes.- Pharmacological Modulation of Glycosphingolipid Metabolism.- Molecular Dynamics of Gangliosides.- Determination of Glycolipid Ligands of Carbohydrate-Binding Proteins Directly on Thin Layer Chromatograms.- Identification of KDN-Gangliosides.- Glycoreplica Peptides.
Details
Erscheinungsjahr: 2018
Fachbereich: Biophysik
Genre: Biologie, Importe
Rubrik: Naturwissenschaften & Technik
Medium: Buch
Inhalt: xv
456 S.
45 s/w Illustr.
56 farbige Illustr.
456 p. 101 illus.
56 illus. in color.
ISBN-13: 9781493985517
ISBN-10: 1493985515
Sprache: Englisch
Einband: Gebunden
Autor: Sonnino, Sandro
Prinetti, Alessandro
Redaktion: Prinetti, Alessandro
Sonnino, Sandro
Herausgeber: Sandro Sonnino/Alessandro Prinetti
Auflage: 1st edition 2018
Hersteller: Springer US
Springer New York
Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße: 260 x 183 x 31 mm
Von/Mit: Alessandro Prinetti (u. a.)
Erscheinungsdatum: 21.06.2018
Gewicht: 1,078 kg
Artikel-ID: 111799935
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