Includes cutting-edge methods and protocols for the study of lipases and phospholipases

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Part 1. Overview of Lipases, Phospholipases and Esterases.- Lipases: An Overview.- Carbohydrate Esterases: An Overview.- Phospholipases: An Overview.- Part 2. Activity Screening and Expression.- Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates.- A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities using ¿-Eleostearic Acid-Containing Substrates.- Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline.- Lipase and Phospholipase Activity Methods for Marine Organisms.- Plant Lipases: Functional Expression of CPLIP1 from Carica papaya.- Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2.- Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies.- Part 3. Optimization of the Biocatalyst Production and Performances.- Recent Advances in Pichia pastoris as Host for Heterologous Expression Systems for Lipases (Review).- Solid-State Fermentation as an Economic Production Method of Lipases and Esterases.- Synthetic Biology to Improve the Production of Lipases and Esterases (Review).- Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases.- Part 4. Applications of Lipases, Phospholipases and Esterases.- Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides.- Targeting Phospholipase D genetically and Pharmacologically for Studying Leukocyte Function.- Lipase-Catalyzed Synthesis of Structured Lipids.- Lipase-Catalyzed Acetylation and Esterification of Bile Acids.- Lipases in Green Chemistry: Deep Eutectic Solvents (DES) as New Green Solvents.- Chemoenzymatic Synthesis of Nitrogen Polymers with Biomedical Applications Catalyzed by Lipases.- Lipases and Esterases as Biocatalyst for Biodiesel Production (Review).- Lipases, Phospholipases andEsterases Biosensors (Review).