Ulf Andersson Vang Ørom: miRNA Biogenesis

Cover: 9781493986231 | miRNA Biogenesis | Methods and Protocols | Ulf Andersson Vang Ørom

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Sprache: Englisch

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Beschreibung

This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic. ¿

Zusammenfassung

Includes cutting-edge methods and protocols for the study of miRNA biogenesis

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

High-throughput characterization of primary microRNA transcripts.- Identifying pri-miRNA transcription start sites.- Metabolic pulse-chase RNA labelling for pri-miRNA processing dynamics.- In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-way System for RNAP II Transcription/Splicing/microRNA Processing.- Purification of Microprocessor-associated factors.- Arresting pri-miRNA processing with Target Site Blockers.- MicroRNA analysis using the quantitative real-time PCR reaction.- MicroRNA analysis using next-generation sequencing.- Identification of microRNA precursor-associated proteins.- Analysis of 3' end modifications in (precursor-) microRNAs by high-throughput.- Uncovering the stability of mature miRNAs by 4-thio-uridine metabolic labeling.- Detection of microRNA-target interactions by Chimera PCR (ChimP).- High-quality overlapping paired-end reads for the detection of A-to-I editing on small RNA.- Targeting miRNA for therapeutics using a Micronome based method for identification of miRNA-mRNA pairs and validation of key regulator miRNA.- Method for detection of miRNAs in non-model organisms with unreported database.- Detection and Verification of Mammalian Mirtrons by Northern Blotting.- Detecting agotrons in Ago CLIPseq data.

Erscheinungsjahr:	2018
Fachbereich:	Andere Fachgebiete
Genre:	Importe , Medizin
Rubrik:	Wissenschaften
Medium:	Buch
Reihe:	Methods in Molecular Biology
Inhalt:	xi 238 S. 13 s/w Illustr. 45 farbige Illustr. 238 p. 58 illus. 45 illus. in color.
ISBN-13:	9781493986231
ISBN-10:	1493986236
Sprache:	Englisch
Herstellernummer:	978-1-4939-8623-1
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Ørom, Ulf Andersson Vang
Herausgeber:	Ulf Andersson Vang Ørom
Auflage:	1st ed. 2018
Hersteller:	Springer US Springer New York Methods in Molecular Biology
Verantwortliche Person für die EU:	Springer Verlag GmbH, Tiergartenstr. 17, D-69121 Heidelberg, juergen.hartmann@springer.com
Maße:	260 x 183 x 20 mm
Von/Mit:	Ulf Andersson Vang Ørom
Erscheinungsdatum:	30.06.2018
Gewicht:	0,678 kg

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