Includes cutting-edge methods and protocols for the assessment of mitochondrial function

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Preparation of 'Functional' Mitochondria: A Challenging Business.- Isolation and Quality Control of Functional Mitochondria.- Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells.- Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels.- Optical Microwell Arrays for Large Scale Studies of Single Mitochondria Metabolic Responses.- Deep Resequencing of Mitochondrial DNA.- Single Cell Analysis of Mitochondrial DNA.- A High-Throughput Next-Generation Sequencing Assay for the Mitochondrial Genome.- Rapid Mitochondrial DNA Isolation Method for Direct Sequencing.- Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR Based Method.- Mitochondria-Targeted RNA Import.- Accurate Measurement of Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR.- mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution.- Simultaneous Quantification of Mitochondrial ATP and ROS Production.- Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine.- Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid.- Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells.- Novel Methods for Measuring the Mitochondrial Membrane Potential.- High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes.- In Vivo Assessment of Mitochondrial Oxygen Consumption.- Imaging Mitochondrial Hydrogen Peroxide in Living Cells.- Simultaneous High-Resolution Measurement of Mitochondrial Respirationand Hydrogen Peroxide Production.- Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells.- Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry.- Assessing the Bioenergetic Profile of Human Pluripotent Stem Cells.- Integrative Methods for Studying Cardiac Energetics.- Computer-Based Prediction of Mitochondria-Targeting Peptides.- Prediction of Mitochondrial Protein Function by Comparative Physiology and Phylogenetic Profiling.- Assessment of Posttranslational Modification of Mitochondrial Proteins.- Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway.- High-Resolution Melting Analysis for Identifying Sequence Variations in Nuclear Genes for Assembly Factors and Structural Subunits of Cytochrome C Oxidase.- Heterologous Inferential Analysis (HIA) as a Method to Understand the Role of Mitochondrial rRNA Mutations in Pathogenesis.- Analysis of Mitochondrial Dysfunction During Cell Death.- The Use of FLIM-FRET for the Detection of Mitochondria-Associated Protein Interactions.- Assessment of Mitochondrial Ca2+ Uptake.- Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS.- Characterization of Mitochondrial Populations During Stem Cell Differentiation.- An Ex Vivo Model for Studying Mitochondrial Trafficking in Neurons.