Peptide Synthesis and Applications

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Sprache: Englisch

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Beschreibung

Peptides are used ubiquitously for studies in biology, biochemistry, chemical biology, peptide based medicinal chemistry, and many other areas of research. There is a number of marketed peptide drugs, and the prospects for the development of new peptide drugs are very encouraging. The second edition of Peptide Synthesis and Applications expands upon the previous editions with current, detailed methodologies for peptide synthesis. With new chapters on laboratory protocols for both the specialist and the non-specialist. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Peptide Synthesis and Application, Second Edition seeks to aid scientists in understanding different approaches to the synthesis of peptides by using a broad range of methods and strategies.

Zusammenfassung

Practical methodologies for peptide synthesis

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: [...]

Inhaltsverzeichnis

Solid-Phase Peptide Synthesis: An Introduction.- Linkers, Resins and General Procedures for Solid-phase Peptide Synthesis.- Peptide Release, Side-chain Deprotection, Work-up, and Isolation.- Synthesis of Peptides using Tert-butyloxycarbonyl (Boc) as the ¿-amino Protection Group.- Sequential Formation of Regioselective Disulfide Bonds in Synthetic Peptides with Multiple Disulfide Bonds.- Synthesis of Cyclic Disulfide-Rich Peptides.- Preparation of C-terminally Modified Chemokines by Expressed Protein Ligation.- Synthesis of C-terminal Peptide Thioesters using Fmoc Based Solid-phase Peptide Chemistry.- Backbone Amide Linker Strategy -Protocols for the Synthesis of C¿terminal Peptide Aldehydes.- Synthesis of N-methylated Peptides: On-resin Methylation and Microwave-Assisted Couplings.- Synthesis of Antimicrobial Peptoids.- Synthesis of Lipidated Peptides.- Solid-Phase Synthesis of Phosphopeptides.- Synthesis of O-Glycopeptides and Construction of Glycopeptide Microarrays.- Instruments for Automated Peptide Synthesis.- Microwave-Assisted Solid-Phase Peptide Synthesis using the Biotage Syro Wave(TM).- Microwave-Assisted Solid-Phase Peptide Synthesis Based on the Fmoc Protecting Group Strategy (CEM).

Details

Erscheinungsjahr:	2013
Fachbereich:	Biophysik
Genre:	Biologie
Rubrik:	Naturwissenschaften & Technik
Medium:	Buch
Reihe:	Methods in Molecular Biology
Inhalt:	x 253 S. 68 s/w Illustr. 253 p. 68 illus.
ISBN-13:	9781627035439
ISBN-10:	1627035435
Sprache:	Englisch
Herstellernummer:	80036415
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Jensen, Knud J. Pedersen, Søren L. Tofteng Shelton, Pernille
Herausgeber:	Knud J Jensen/Pernille Tofteng Shelton/Søren L Pedersen
Auflage:	2nd ed. 2013
Hersteller:	Humana Press Humana Press Inc. Methods in Molecular Biology
Maße:	260 x 183 x 20 mm
Von/Mit:	Knud J. Jensen (u. a.)
Erscheinungsdatum:	14.08.2013
Gewicht:	0,7 kg

Artikel-ID: 105948940