Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Antibody phage display.- Construction of human immune and naive scFv Phage Display libraries.- Construction of Naïve and Immune Human Fab Phage Display Library.- Construction of synthetic antibody phage display libraries.- Construction of chicken and ostrich antibody libraries.- Construction of Rabbit Immune Antibody Libraries.- Isolation and characterization of single-domain antibodies from immune phage display libraries.- Phagekines: directed evolution and characterization of functional cytokines displayed on phages.- Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly.- Construction of an ultra-large phage display library by Kunkel mutagenesis and rolling circle amplification.- Construction of semi-synthetic shark vNAR yeast surface display antibody libraries.- Antibody selection via Phage Display in microtiter plates.- Antibody selection in solution using magnetic beads.- Streptavidin Coated Solid Phase Extraction (SPE) TipsFor Antibody Phage Display Biopanning.- Magnetic Nanoparticle-Based Semi-Automated Panning for High-throughput Antibody Selection.- Antibody selection on cells targeting membrane proteinsTargeting Intracellular Antigens With pMHC-binding Antibodies: A Phage Display Approach.- Antibody Isolation from a Human Synthetic Libraries of Single-chain Antibodies and analysis using NGS.- Selection of affibody affinity proteins from phagemid libraries.- Antibody affinity and stability maturation by error-prone PCR.- Antibody batch cloning.- Deep Mining of Complex Antibody Phage Pools.- High-throughput IgG reformatting and expression using hybrid secretion signals and InTag positive selection technology.- Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae Protocols.- Mapping polyclonal antibody responses to infection using next generation phage display.- Applications of high-throughput DNA sequencing to single-domain antibodydiscovery and engineering.- Biomarker discovery by ORFeome Phage Display.- Mapping Epitopes by Phage Display.- Epivolve: A Protocol for Site-directed Antibodies.