Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Protein Engineering - Past, Present, and Future.- (Semi)rational Protein Design.- Computational Analysis of Protein Tunnels and Channels.- YASARA - A Tool to Obtain Structural Guidance in Biocatalytic Investigations.- A Computational Library Design Protocol for Rapid Improvement of Protein Stability - FRESCO.- Directed Evolution of Proteins Based on Mutational Scanning.- A Brief Guide to the High-throughput Expression of Directed Evolution Libraries.- Library Growth and Protein Expression: Optimal and Reproducible Microtiter Plate Expression of Recombinant Enzymes in
E. coli
using MTP Shakers.- Normalized Screening of Protein Engineering Libraries by Split-GFP Crude Cell Extract Quantification.- Functional Analysis of Membrane Proteins Produced by Cell-Free Translation.- Practical Considerations Regarding the Choice of the Best High-throughput Assay.- High-throughput Screening Assays for Lipolytic Enzymes.- Continuous High-throughput Colorimetric Assays for ¿-transaminases.-Colorimetric High-troughput Screening Assays for the Directed Evolution of Fungal Laccases.- Directed Co-evolution of Two Cellulosic Enzymes in
Escherichia coli
Based on their Synergistic Reactions.- Program-guided Design of High-throughput Enzyme Screening Experiments and Automated Data Analysis/Evaluation.- Solid Phase Agar Plate Assay for Screening Amine Transaminases.- Ultrahigh-throughput Screening of Single-cell Lysates for Directed Evolution and Functional Metagenomics.- Isolation of pH-sensitive Antibody Fragments by Fluorescence-activated Cell Sorting and Yeast Surface Display.- Library Generation and Auxotrophic Selection Assays in
Escherichia coli
and
Thermus thermophiles.