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Real-time PCR
Taschenbuch von M Tevfik Dorak
Sprache: Englisch

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Beschreibung
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Über den Autor

Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK

Inhaltsverzeichnis

1. An Introduction to Real-time PCR 2. Data Analysis and Reporting 3. Relative Quantification 4. Normalisation 5. High-Throughput Primer and Probe Design 6. Quantitative Analysis of Ocular Gene Expression 7. Quantitative Gene Expression by Real-time PCR 8. Real-time PCR Using SYBR Green 9. High-Resolution Melting Analysis for Scanning and Genotyping 10. Quantitative Analyses of DNA Methylation 11. Mitochondrial DNA Analysis 12. Real-time Immuno-PCR 13. Clinical Microbiology 14. Clinical Virology 15. Solid Organ Transplant Monitoring 16. Real-time PCR Applications in Haematology 17. Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes - the Example of the Haemoglobinopathies

Details
Erscheinungsjahr: 2006
Fachbereich: Biophysik
Genre: Biologie, Importe
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
ISBN-13: 9780415377348
ISBN-10: 041537734X
Sprache: Englisch
Einband: Kartoniert / Broschiert
Redaktion: Dorak, M Tevfik
Hersteller: Taylor & Francis
Verantwortliche Person für die EU: Libri GmbH, Europaallee 1, D-36244 Bad Hersfeld, gpsr@libri.de
Maße: 244 x 170 x 20 mm
Von/Mit: M Tevfik Dorak
Erscheinungsdatum: 27.04.2006
Gewicht: 0,625 kg
Artikel-ID: 128392013
Über den Autor

Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK

Inhaltsverzeichnis

1. An Introduction to Real-time PCR 2. Data Analysis and Reporting 3. Relative Quantification 4. Normalisation 5. High-Throughput Primer and Probe Design 6. Quantitative Analysis of Ocular Gene Expression 7. Quantitative Gene Expression by Real-time PCR 8. Real-time PCR Using SYBR Green 9. High-Resolution Melting Analysis for Scanning and Genotyping 10. Quantitative Analyses of DNA Methylation 11. Mitochondrial DNA Analysis 12. Real-time Immuno-PCR 13. Clinical Microbiology 14. Clinical Virology 15. Solid Organ Transplant Monitoring 16. Real-time PCR Applications in Haematology 17. Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes - the Example of the Haemoglobinopathies

Details
Erscheinungsjahr: 2006
Fachbereich: Biophysik
Genre: Biologie, Importe
Rubrik: Naturwissenschaften & Technik
Medium: Taschenbuch
ISBN-13: 9780415377348
ISBN-10: 041537734X
Sprache: Englisch
Einband: Kartoniert / Broschiert
Redaktion: Dorak, M Tevfik
Hersteller: Taylor & Francis
Verantwortliche Person für die EU: Libri GmbH, Europaallee 1, D-36244 Bad Hersfeld, gpsr@libri.de
Maße: 244 x 170 x 20 mm
Von/Mit: M Tevfik Dorak
Erscheinungsdatum: 27.04.2006
Gewicht: 0,625 kg
Artikel-ID: 128392013
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