Metabolic Flux Analysis in Eukaryotic Cells

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Beschreibung

This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

Preface...

Table of Contents...

Contributing Authors...

1. Determination of Isotopologue and Tandem Mass Isotopologue Fractions using Gas Chromatography Chemical Ionization Time of Flight Mass Spectrometry: Methodology and Uncertainty of Measurement

Teresa Mairinger and Stephan Hann

2. Non-Targeted Mass Isotopolome Analysis using Stable Isotope Patterns to Identify Metabolic Changes

Christian-Alexander Dudek, Lisa Schlicker, and Karsten Hiller

3. Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C Meabolic Flux Analysis

Damini Jaiswal, Anjali Mittal, Deepak Nagrath, and Pramod P. Wangikar

4. Deuterium Tracing to Interrogate Compartment-Specific NAD(p)H Metabolism in Cultured Mammalian Cells

Esther W. Lim, Seth J. Parker, and Christian M. Metallo

5. Large-Scale Profiling of Cellular Metabolic Activities using Deep 13C Labeling Medium

Nina Grankvist, Jeramie D. Watrous, Mohit Jain, and Roland Nilsson

6. Analyzing the Metabolism of Metastases in Mice

Patricia Altea-Manzano, Dorien Broekaert, João A.G. Duarte, Juan Fernández-García, Mélanie Planque, and Sarah-Maria Fendt

7. Robusy Analytical methods for the Accurate Quantification of the Total Biomass Composition of Mammalian Cells

Diana Széliová, Harald Schoeny, spela Knez, Christina Troyer, Cristina Coman, Evelyn Rampler, Gunda Koellensperger, Robert Ahrends, Stephen Hann, Nicole Borth, Jürgen Zanghellini, and David E. Ruckerbauer

8. Quantifying Nitric Oxide Flux Distributions

Darshan M. Sivaloganathan, Xuanqing Wan, and Mark P. Brynildsen

9. SWATH: A Data-Independent Tandem Mass Spectrometry Method to Quantify 13C Enrichment in Cellular Metabolites and Fragments

Damini Jaiswal and pramod P. Wangikar

10. Quantifying Metabolic Transfer Mediated by Extracellular Vesicles using Exo-MFA: An Integrated Empirical and COmputational Platform

Abhinav Achrejal, Noah Meurs, and Deepak Nagrath

11. Comparative Metabolic Network Flux Analysis to Identify Differences in Cellular Metabolism

Sarah McGarrity, Sigurður T. Karvelsson, Ólafur E. Sigurjónsson, and Óttar Rolfsson

12. Software Supporting a Workflow of Quantitative Dynamic Flux Maps Estimation in Central Metabolism from SIRM Experimental Data

Vitaly A. Selivanov, Silvia Marin, Josep Tarragó-Celada, Andrew N. Lane, Richard M. Higashi, Teresa W-M Fan, Pedro de Atauri, and Marta Cascante

13. Inferring Metabolic Flux from Time-Course Metabolomics

Scott Campit and Sriram Chandrasekaran

14. Using Metabolic Network Reconstructions to Predict Drug Targets and Off-Target Effects

Kristopher Rawls, Bonnie V. Dougherty, and Jason Papin

15. Single-Cell Digital Twins for Cancer Preclinical Investigation

Marzia Di Filippo, Chiara Damiani, Marco Vanoni, Davide Maspero, Giancarlo Mauri, Lilia Alberghina, and Dario Pescini

16. Supply and Demand Analysis of Autophagy

André du Toit, Ben Loos, and Jan Hendrik S. Hofmeyr

17. Thermodynamic Approaches in Flux Analysis

Sabine Peres and Vincent Fromion

Details

Erscheinungsjahr:	2021
Fachbereich:	Allgemeines
Genre:	Biologie
Rubrik:	Naturwissenschaften & Technik
Medium:	Taschenbuch
Reihe:	Methods in Molecular Biology
Inhalt:	xiii 370 S. 23 s/w Illustr. 64 farbige Illustr. 370 p. 87 illus. 64 illus. in color.
ISBN-13:	9781071601617
ISBN-10:	107160161X
Sprache:	Englisch
Ausstattung / Beilage:	Paperback
Einband:	Kartoniert / Broschiert
Redaktion:	Nagrath, Deepak
Herausgeber:	Deepak Nagrath
Auflage:	1st ed. 2020
Hersteller:	Springer US Springer US, New York, N.Y. Methods in Molecular Biology
Maße:	254 x 178 x 21 mm
Von/Mit:	Deepak Nagrath
Erscheinungsdatum:	26.08.2021
Gewicht:	0,721 kg

Artikel-ID: 119467213