Germain Rousselet: Macrophages

Cover: 9781493978366 | Macrophages | Methods and Protocols | Germain Rousselet | Buch | xii

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Beschreibung

This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.

The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.[...].

Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.

Über den Autor

Zusammenfassung

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhaltsverzeichnis

Culture of Human Monocyte-Derived Macrophages.- A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages.- Growing Murine Bone Marrow-Derived Macrophages.- Immortalization of Murine Bone Marrow-Derived Macrophages.- Isolation and Identification of Murine Serous Cavity Macrophages.- Isolation and Identification of Interstitial Macrophages from the Lungs using Different Digestion Enzymes and Staining Strategies.- Isolation and Phenotyping of Adult Mouse Microglial Cells.- Isolation and Phenotyping of Bone Marrow Macrophages.- Isolation and Phenotyping of Intestinal Macrophages.- Phenoytyping Tumor Associated Macrophages.- Activating Murine Macrophages In Vitro.- Polarizing Macrophages In Vitro.- Viral Replication Assay in Bone Marrow-Derived Macrophages.- Macrophage Bactericidal Assays.- Quantitative Phagocytosis Assays in Primary and Cultured Macrophages.- Observing Frustrated Phagocytosis and Phagosome Formation and Closure using Total Internal Reflection Fluorescence Microscopy (TIRFM).- Chromatine Immunoprecipitation in Macrophages.- Transfecting Macrophages.- In Vitro Migration Assays.- Cytokine-Induced Acute Inflammatory Monarticular Arthritis.- Characterizing Activation, Proliferation, and Ontogeny of Murine Macrophages in Parasitic Helminth Infections.- Genetic Models of Macrophage Depletion.- Depleting Macrophages In Vivo with Clodronate-Liposomes.- Cre Driver Mice Targeting Macrophages.

Details

Erscheinungsjahr:	2018
Fachbereich:	Allgemeines
Genre:	Biologie
Rubrik:	Naturwissenschaften & Technik
Thema:	Lexika
Medium:	Buch
Reihe:	Methods in Molecular Biology
Inhalt:	xii 278 S. 8 s/w Illustr. 35 farbige Illustr. 278 p. 43 illus. 35 illus. in color.
ISBN-13:	9781493978366
ISBN-10:	1493978365
Sprache:	Englisch
Herstellernummer:	978-1-4939-7836-6
Ausstattung / Beilage:	HC runder Rücken kaschiert
Einband:	Gebunden
Redaktion:	Rousselet, Germain
Herausgeber:	Germain Rousselet
Auflage:	1st ed. 2018
Hersteller:	Springer US Springer New York Methods in Molecular Biology
Maße:	260 x 183 x 21 mm
Von/Mit:	Germain Rousselet
Erscheinungsdatum:	15.05.2018
Gewicht:	0,817 kg

Artikel-ID: 111302057